Fig. 3

Pharmacogenomic interactions in gastric cancer. a Volcano plot representation of pharmacogenomic interactions in gastric cancer with fold-change drug comparison (x-axis) and its significance (y-axis). Each node represents a single genomic alteration-drug interaction, and the size is proportional to the number of tumors with the respective genomic variation. b Violin plots of drug AUC values for tumors with thegenomic alteration compared to those without from selected gene-drug interactions. Horizontal lines within the violin plots represent 0.25, 0.50, and 0.75 quantiles. c Box plots of AZD5363 AUC values among tumors with different PIK3CA variations. Box plots span from the first to third quartiles, and the whiskers represent the 1.5 interquartile range. d Cell proliferation assay of gastric cancer cell-lines. e Effects of AZD5363 on the PI3K/AKT/mTOR signaling pathway in gastric cancer cell-lines with different mutations of PIK3CA or the wild-type gene. f Scatter plot of AZD5363 AUCs in our cohort (left panel). The AUC of the PDC that was isolated from the indicated patient (right panel) is highlighted in a red circle. Dotted green and orange horizontal lines represent relative resistance and sensitivity, respectively. T₁-weighted contrast-enhanced magnetic resonance images of tumor samples from the gastric cancer patient who received AZD5363 treatment. The red arrow indicates measurable or progressed tumor; the orange arrow represents partial response. P values in a, b were derived from two-sided Wilcoxon rank-sum tests, the P value in c from one-way ANOVA