Table 2 Summary of all RT-PCR primers and PCR protocols used
From: Cell-free DNA analysis reveals POLR1D-mediated resistance to bevacizumab in colorectal cancer
Gene | Forward primer sequence (5′ → 3′) | Reverse primer sequence (5′ → 3′) | PCR protocol | Cycles |
|---|---|---|---|---|
FLT3 | TTTCACAGGACTTGGACAGAGATTT | GAGTCCGGGTGTATCTGAACTTCT | 95 °C/50 °C/72 °C | 40 |
VIM | GAACTTTGCCGTTGAAGCTG | TCTCAATGTCAAGGGCCATC | 95 °C/56 °C/73 °C | 40 |
CDH1 | GTCAGGTGCCTGAGAACG | GCCATCGTTGTTCACTGG | 95 °C/56 °C/73 °C | 40 |
POLR1D (V1) | CCACCTGAGGATCCAGAAAC | CCTCGTGCAATACAAATGTCA | 95 °C/60 °C | 40 |
POLR1D (V2) | AAGAACTGCTTAAGGAGGCAA | TCTTCGCTGGTTCCTTATCG | 95 °C/60 °C | 40 |
PAN3 | TTGGTGCCCTCAACATCTCT | TTGATCCCATCGGAACTAGC | 95 °C/60 °C | 40 |
CDX2 | GAACCTGTGCGAGTGGATG | TCCTCCGGATGGTGATGTAG | 95 °C/60 °C | 40 |
PDX1 | CCTTTCCCATGGATGAAGTC | TTCAACATGACAGCCAGCTC | 95 °C/60 °C | 40 |
LNX2 | ATGCAACGTTGTGATCTGGA | CCAAACAGTCTGCTTCTGGA | 95 °C/60 °C | 40 |
GAPDH | CCAAAATCAAGTGGGGCGATG | AAAGGTGGAGGAGTGGGTGTCG | 95 °C/60 °C | 40 |