SARS-CoV-2 vaccine ChAdOx1 nCoV-19 infection of human cell lines reveals low levels of viral backbone gene transcription alongside very high levels of SARS-CoV-2 S glycoprotein gene transcription

Table 1 Read counts for individual dRNAseq datasets

Sample	Total reads	Longest read	Average read length	Mapped to human transcriptome	Mapped to Chadox1 nCoV-19 (E1 region in 293)	ChAdOx1 nCoV-19 mapped reads as a percentage of human mapped reads (E1 region in 293)
MRC5 mock	987,540	23,008	1607	836,380
MRC5 24hpi	633,046	23,426	1600	578,180	6190	1.07
MRC5 48hpi	903,254	32,925	1549	810,640	13,547	1.67
MRC5 72hpi	1,020,009	26,592	1552	758,197	9723	1.28
A549 mock	1,171,427	17,654	1270	844,383
A549 24hpi	1,411,415	22,536	1381	1,243,531	3255	0.26
A549 48hpi	1,771,635	25,111	1287	1,356,800	3194	0.24
A549 72hpi	1,790,983	19,641	1312	1,486,051	2620	0.18
293 24hpi	1,013,425	31,829	1482	768,842	109,197 (+ 1014)	14.20 (+ 0.13)

The total number of reads, longest read and average read length is noted for each dataset. In addition, the table details how many reads mapped to the human transcriptome list, the ChAdOx1 nCoV-19 genome and (in the case of HEK293 cell dataset) the E1/pIX region from human adenovirus type 5 known to be cloned into the cell line. Also shown is the percent contribution of the mapped reads relative to the total number of reads analysed. In the case of reads mapping to the ChAdOx1 nCoV-19 genome from the HEK293 cell experiment, the figures in brackets refer solely to reads mapping to the adenovirus type 5 sequences

ISSN: 1756-994X