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Table 1 Read counts for individual dRNAseq datasets

From: SARS-CoV-2 vaccine ChAdOx1 nCoV-19 infection of human cell lines reveals low levels of viral backbone gene transcription alongside very high levels of SARS-CoV-2 S glycoprotein gene transcription

Sample Total reads Longest read Average read length Mapped to human transcriptome Mapped to Chadox1 nCoV-19 (E1 region in 293) ChAdOx1 nCoV-19 mapped reads as a percentage of human mapped reads (E1 region in 293)
MRC5 mock 987,540 23,008 1607 836,380   
MRC5 24hpi 633,046 23,426 1600 578,180 6190 1.07
MRC5 48hpi 903,254 32,925 1549 810,640 13,547 1.67
MRC5 72hpi 1,020,009 26,592 1552 758,197 9723 1.28
A549 mock 1,171,427 17,654 1270 844,383   
A549 24hpi 1,411,415 22,536 1381 1,243,531 3255 0.26
A549 48hpi 1,771,635 25,111 1287 1,356,800 3194 0.24
A549 72hpi 1,790,983 19,641 1312 1,486,051 2620 0.18
293 24hpi 1,013,425 31,829 1482 768,842 109,197 (+ 1014) 14.20 (+ 0.13)
  1. The total number of reads, longest read and average read length is noted for each dataset. In addition, the table details how many reads mapped to the human transcriptome list, the ChAdOx1 nCoV-19 genome and (in the case of HEK293 cell dataset) the E1/pIX region from human adenovirus type 5 known to be cloned into the cell line. Also shown is the percent contribution of the mapped reads relative to the total number of reads analysed. In the case of reads mapping to the ChAdOx1 nCoV-19 genome from the HEK293 cell experiment, the figures in brackets refer solely to reads mapping to the adenovirus type 5 sequences