|
Sample
|
Total reads
|
Longest read
|
Average read length
|
Mapped to human transcriptome
|
Mapped to Chadox1 nCoV-19 (E1 region in 293)
|
ChAdOx1 nCoV-19 mapped reads as a percentage of human mapped reads (E1 region in 293)
|
|---|
|
MRC5 mock
|
987,540
|
23,008
|
1607
|
836,380
| | |
|
MRC5 24hpi
|
633,046
|
23,426
|
1600
|
578,180
|
6190
|
1.07
|
|
MRC5 48hpi
|
903,254
|
32,925
|
1549
|
810,640
|
13,547
|
1.67
|
|
MRC5 72hpi
|
1,020,009
|
26,592
|
1552
|
758,197
|
9723
|
1.28
|
|
A549 mock
|
1,171,427
|
17,654
|
1270
|
844,383
| | |
|
A549 24hpi
|
1,411,415
|
22,536
|
1381
|
1,243,531
|
3255
|
0.26
|
|
A549 48hpi
|
1,771,635
|
25,111
|
1287
|
1,356,800
|
3194
|
0.24
|
|
A549 72hpi
|
1,790,983
|
19,641
|
1312
|
1,486,051
|
2620
|
0.18
|
|
293 24hpi
|
1,013,425
|
31,829
|
1482
|
768,842
|
109,197 (+ 1014)
|
14.20 (+ 0.13)
|
- The total number of reads, longest read and average read length is noted for each dataset. In addition, the table details how many reads mapped to the human transcriptome list, the ChAdOx1 nCoV-19 genome and (in the case of HEK293 cell dataset) the E1/pIX region from human adenovirus type 5 known to be cloned into the cell line. Also shown is the percent contribution of the mapped reads relative to the total number of reads analysed. In the case of reads mapping to the ChAdOx1 nCoV-19 genome from the HEK293 cell experiment, the figures in brackets refer solely to reads mapping to the adenovirus type 5 sequences
