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Table 2 List of ORFs searched for and the transcript frequency for each ORF

From: SARS-CoV-2 vaccine ChAdOx1 nCoV-19 infection of human cell lines reveals low levels of viral backbone gene transcription alongside very high levels of SARS-CoV-2 S glycoprotein gene transcription

 

MRC-5 cells

A549 cells

293 cells

Feature

Count 24 h

Percent of total at 24 h

Count 48 h

Percent of total at 48 h

Count 72 h

Percent of total at 72 h

Count 24 h

Percent of total at 24 h

Count 48 h

Percent of total at 48 h

Count 72 h

Percent of total at 72 h

Count 24 h

Percent of total at 24 h

Total of all reads

4443

100.00

9201

100.00

6849

100.00

2070

100.00

1937

100.00

1811

100.00

62,281

100.00

SARS_CoV_2_S_protein

4156

93.54

8570

93.14

6453

94.22

1536

74.20

1447

74.70

1645

90.83

10,586

17.00

None from list

148

3.33

278

3.02

214

3.12

92

4.44

112

5.78

53

2.93

4138

6.64

pIX

21

0.47

27

0.29

23

0.34

14

0.68

13

0.67

2

0.11

909

1.46

DBP(E2A)

1

0.02

3

0.03

0

0.00

205

9.90

95

4.90

13

0.72

5198

8.35

fibre(L5)

0

0.00

0

0.00

0

0.00

2

0.10

9

0.46

2

0.11

6011

9.65

hexon(L3)

0

0.00

0

0.00

0

0.00

16

0.77

20

1.03

3

0.17

3990

6.41

33K_full_length(L4)

0

0.00

0

0.00

0

0.00

10

0.48

20

1.03

7

0.39

3217

5.17

preVII(L2)

0

0.00

0

0.00

0

0.00

12

0.58

7

0.36

2

0.11

3212

5.16

100K(L4)

0

0.00

0

0.00

0

0.00

11

0.53

25

1.29

7

0.39

2212

3.55

i_leader_protein

0

0.00

0

0.00

0

0.00

18

0.87

46

2.37

11

0.61

1969

3.16

IVa2

0

0.00

0

0.00

0

0.00

6

0.29

14

0.72

3

0.17

1676

2.69

pV(L2)

0

0.00

0

0.00

0

0.00

4

0.19

3

0.15

0

0.00

1373

2.20

52/55K(L1)

0

0.00

0

0.00

0

0.00

20

0.97

28

1.45

6

0.33

1345

2.16

22K(L4)

0

0.00

0

0.00

0

0.00

9

0.43

9

0.46

2

0.11

1193

1.92

preX(L2)

0

0.00

0

0.00

0

0.00

6

0.29

8

0.41

4

0.22

1180

1.89

E4_orf2

0

0.00

2

0.02

0

0.00

0

0.00

1

0.05

2

0.11

1040

1.67

E4_orf3

0

0.00

1

0.01

0

0.00

13

0.63

8

0.41

3

0.17

1011

1.62

preVI(L3)

0

0.00

0

0.00

0

0.00

8

0.39

7

0.36

1

0.06

821

1.32

preVIII(L4)

0

0.00

0

0.00

0

0.00

1

0.05

2

0.10

1

0.06

821

1.32

penton_base(L2)

0

0.00

0

0.00

0

0.00

1

0.05

2

0.10

1

0.06

631

1.01

Ad5_E1b_19K

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

506

0.81

E4_orf1

0

0.00

0

0.00

0

0.00

1

0.05

0

0.00

0

0.00

286

0.46

preIIIa(L1)

0

0.00

0

0.00

0

0.00

5

0.24

3

0.15

0

0.00

239

0.38

E4_orf4

0

0.00

0

0.00

0

0.00

2

0.10

2

0.10

0

0.00

187

0.30

UXP

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

159

0.26

Ad5_E1a_13S

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

97

0.16

E4_orf6_7

0

0.00

0

0.00

0

0.00

1

0.05

0

0.00

1

0.06

79

0.13

23K_protease(L3)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

70

0.11

Ad5_E1a_12S

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

26

0.04

preTP(E2B)

0

0.00

0

0.00

0

0.00

1

0.05

0

0.00

0

0.00

23

0.04

Ad5_pIX

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

10

0.02

pol(E2B)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

4

0.01

Ad5_E1a_10S(171R)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

3

0.00

E4_orf6

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

3

0.00

Ad5_E1b_13S(E1b-84R)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

2

0.00

Ad5_E1b_22S(E1b 55 K)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

2

0.00

Ad5_E1a_9S

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

1

0.00

Ad5_E1a_11S(217R)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

Ad5_E1b_14.5S(E1b-93R)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

Ad5_E1b_14S(E1b-156R)

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

Ad5_E1b_novel-84R

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

0

0.00

  1. This table details how many transcripts would code for each indicated ORF from the ChAdOx1 nCoV-19 genome or ORFs coded by the region of Ad5 integrated into the HEK293 cell line. The “total of all reads” row indicates how many transcripts map to either the ChAdOx nCoV-19 genome or the region of human Ad5 integrated into the HEK 293 genome. The “none from list” row indicates how many transcripts did map but the pipeline was unable to correlate the 5′ most ORF on that transcript with any of the known ORFs from the genomes under consideration