SARS-CoV-2 vaccine ChAdOx1 nCoV-19 infection of human cell lines reveals low levels of viral backbone gene transcription alongside very high levels of SARS-CoV-2 S glycoprotein gene transcription

Table 3 Counts of transcripts arising from the expected transcription start site for S glycoprotein transcripts and their assignment

Cell line	Number of transcripts starting at TSS for S glycoprotein	Number of transcripts that code for S glycoprotein (percentage)	Number of transcripts belonging to the dominant transcript class (percentage)
MRC-5	20,471	19,187 (93.7%)	9746 (47.6%)
A549	4886	4607 (94.3%)	2422 (49.6%)
293	11,094	10,586 (95.4%)	6050 (54.5%)

This table details numbers of transcripts which map to the expected transcription start site for the major S glycoprotein transcript at 1700 nt. For each cell line, the counts are the accumulated numbers of transcripts at all the time points examined and the numbers (and percentage) of transcripts that should code for the S glycoprotein as the 5′ most ORF

ISSN: 1756-994X