Fig. 3From: Development of double-positive thymocytes at single-cell resolutionLy6d and CD2 can serve as new markers to gate DP subtypes. a Heat map of selected cell surface marker genes (color-coded by clusters). Columns denote cells; rows denote genes. b Violin plots showing normalized expression levels of selected marker genes that changed during the course of T cell development (Cd8a, Ly6d, Cd2 and Mki67). c Flow cytometry measurements of Ly6d expression in thymocyte subpopulations. d Flow cytometry gating strategy for identifying ISPs and the DP subset. e Flow cytometry measurement of CD24, CD3, Rorγt and CD69 expression in ISPs and the DP subtypeBack to article page