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Fig. 3 | Genome Medicine

Fig. 3

From: Development of double-positive thymocytes at single-cell resolution

Fig. 3

Ly6d and CD2 can serve as new markers to gate DP subtypes. a Heat map of selected cell surface marker genes (color-coded by clusters). Columns denote cells; rows denote genes. b Violin plots showing normalized expression levels of selected marker genes that changed during the course of T cell development (Cd8a, Ly6d, Cd2 and Mki67). c Flow cytometry measurements of Ly6d expression in thymocyte subpopulations. d Flow cytometry gating strategy for identifying ISPs and the DP subset. e Flow cytometry measurement of CD24, CD3, RorĪ³t and CD69 expression in ISPs and the DP subtype

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