Fig. 5
From: Development of double-positive thymocytes at single-cell resolution
MHC-I antigen presentation occurred between thymocytes. a Top enriched GO terms for marker genes of DPsels, CD4sps and CD8sps. GO term enrichment analysis was performed with Metascape. b Violin plots showing normalized expression levels of selected marker genes that changed during the course of T cell differentiation (H2-D1, H2-K1, B2 m and Tapbp). c Flow cytometry measurements of MHC-I expression in thymocyte subpopulations. d Immune synapse detection by confocal microscopy. The observed immune synapses are indicated by white arrows. The color bar represents 5 μm. e Gating scheme for thymocyte doublets by ImageStream flow cytometry. f The upper panel shows an example of an immune synapse (indicated with white arrows) formed between adjacent thymocytes. The bottom panel shows an example of adjacent thymocytes with no significant interactions