Fig. 4
From: Microglial PGC-1α protects against ischemic brain injury by suppressing neuroinflammation
PGC-1α attenuates neuroinflammation by suppressing NLRP3 hyperactivation. a Expression of various cytokines/chemokines in microglia by protein array analysis. b FACS analysis of IL-1β, IL-6, and TNF-α levels in microglia from the two types of mice after tMCAO. c Quantification of IL-1β, IL-6, and TNF-α levels based on FACS analysis. d FACS analysis of NLRP3 expression in microglia from the PGC-1αf/f and mPGC-1α mice at 24 h after tMCAO. e Quantification of NLRP3 expression based on FACS. f The mRNA expression of inflammatory cytokines was detected in BV2 cells after LPS stimulation for 6 h. g Quantification of IL-1β, IL-6, and TNF-α levels by ELISA. h Western blot analysis of NLRP3, ASC, and pro-IL-1β in cellular lysates and IL-1β in supernatant. *p < 0.05, **p < 0.01, ***p < 0.001; a n = 3 per group, b–e n = 6 per group, f–h n = 5–10 per group