Fig. 4

PRMT1 and SMARCA4 cooperatively activate TNS4 and EGFR transcription in HCT116 cells. a, b Quantitative real-time PCR analysis of indicated mRNAs normalized to GAPDH (a) and western blot analysis of indicated proteins normalized to histone H4 and Hsp70 (b) from HCT116 cells that had been transfected with EV (empty vector, MSCV) or PRMT1-WT, and transfected or not with a SMARCA4 expression construct. c, d Quantitative real-time PCR analysis of indicated mRNAs normalized to GAPDH (c) and western blot analysis of indicated proteins normalized to histone H4 and Hsp70 (d) from NC or PRMT1-KD in HCT116 cells transfected or not with a SMARCA4 expression construct. e, f Quantitative real-time PCR analysis of indicated mRNAs normalized to GAPDH (e) and western blot analysis of indicated proteins normalized to histone H4 and Hsp70 (f) from HCT116 cells transfected with PRMT-WT or PRMT1-Δ constructs and transfected or not with a SMARCA4 expression construct. For a, c, and e, results are shown as mean ± s.d. from three independent experiments; *P < 0.05, **P < 0.01 compared with the indicated control