Fig. 5

L83V and R176W variants cause increased p62 levels in the thorax in aged flies. a Confocal micrographs of the thorax muscle from 8-week-old flies—dAtad3a-null mutants expressing dAtad3a^WT, dAtad3a^R176W, or dAtad3a^L83V. ATP5A (green) labels mitochondria. Ref (2) P is the Drosophila homolog of p62 (red). Arrows indicate Ref (2) P signals with the absence of ATP5A signals. Scale bars indicate 100 μm. b Western blots for the protein levels of Ref (2) P, ATP5A, and Actin from dAtad3a mutant fly thoraxes expressing dAtad3a^WT, dAtad3a^R176W, or dAtad3a^L83V (n = 10 per genotype). c Quantification of Ref (2) P and d ATP5A level. Ref (2) P and ATP5A were normalized by Actin. Three biological replicates were quantified. Error bars indicate SEM. P values were calculated using Student’s t test. *P < 0.05, **P < 0.01