Fig. 6

L83V and R176W variants cause aberrant mitochondrial morphology, increased autophagic and mitophagic vesicles. a Electron micrographs of the thorax muscles from 8-week-old dAtad3a mutant flies expressing dAtad3a^WT, dAtad3a^R176W, or dAtad3a^L83V. Arrows show autophagosomes (i, ii, and v), autolysosomes (v and viii), lysosomes (vi and ix), and mitophagosomes (iv and vii). Scale bars indicate 600 nm. b Quantification of mitochondria size, mitochondria phenotypes, numbers of autophagosome, autolysosome, lysosome, and mitophagosome. The respective number of the vesicles was normalized by observed area (μm²). Error bars indicate SEM. P values were calculated using Student’s t test. *P < 0.05, ***P < 0.001