Fig. 6
Targeted sequencing of SARS-CoV-2 of clinical samples. Targeted sequencing was performed on a total of 100 clinical samples (30 positive and 70 negative) derived from nasopharyngeal swabs. The ratio of reads aligning to viral and RPP30 sequences is displayed, with samples shown in increasing order of the ratio of reads aligning to SARS-CoV-2 to RPP30. The positive samples (blue; N = 3) were found to be higher than the reads ratio of negative samples by setting a threshold of greater than three times greater than the standard deviation away from the mean of the negative samples (dashed line). Inset: Correlation of viral reads ratio to qPCR CT values as derived from clinical tests (correlation coefficient = − 0.90, p = 2.4e−11)