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Table 2 Sex, genotype, age, and n for each experiment

From: Deletion of a non-canonical regulatory sequence causes loss of Scn1a expression and epileptic phenotypes in mice

Experiment

n

Region

Age

qPCR

WT = 4F, 1b+/− = 5 (4F, 1 M), 1b−/− = 7 (2F, 5 M)

Cortex

Hippocampus

Cerebellum

3 months

Western blot

WT = 3 M, 1b+/− = 3 M, 1b−/− = 3 M

Cortex

Hippocampus

Cerebellum

P29-P32

IF

WT = 3, 1b+/− = 3, 1b−/− = 3

Brain

P28

RNA-seq

WT = 2 M, 1b+/− = 4 (2 M, 2F), 1b−/− = 2F

Forebrain

P7

 

WT = 2F, 1b−/− = 2 (1 M, 1F); WT = 3 M, 1b+/− = 4 (3 M, 1F)

Hippocampus

P32

Behavior

WT = 26(12 M, 14F), 1b+/− = 30(15 M, 15F), 1b−/− = 12 (6 M, 6F)

 

Started at 6 weeks

Thermal-induced seizures

WT = 9(5 M, 4F)1b+/− = 9 (6 M, 3F)

 

P22