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Table 3 qPCR primers

From: Deletion of a non-canonical regulatory sequence causes loss of Scn1a expression and epileptic phenotypes in mice

Target

Sequence (5′–3′)

Human Gapdh

F-CAATGACCCCTTCATTGACC

R-TTGATTTTGGAGGGATCTCG

Mouse Gapdh

F-TCACCACCATGGAGAAGGC

R-GCTAAGCAGTTGGTGGTGCA

Scn1a (mouse and human)

F-CTCGTTCCTGATCGTGTTCC

R-ATCCTGTCCACAGCAATCTG

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Genome Medicine

ISSN: 1756-994X

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