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Fig. 6 | Genome Medicine

Fig. 6

From: Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysis

Fig. 6

Cryopreservation provides high quality immunophenotyping using CITE-Seq. a UMAP visualisation of 2621 cells sequenced from a breast cancer case cryopreserved as CT. Clusters were annotated based on canonical cell type markers by RNA expression. CITE-Seq was performed on this case using a panel of 15 canonical cell type markers. b Heatmap of rescaled antibody-derived tag (ADT) values for relevant markers for cancer/epithelial cells (EPCAM), endothelial cells (CD31/PECAM1 and CD34), perivascular cells (MCAM/CD146 and THY-1/CD90), cancer-associated fibroblasts (THY-1/CD90 and CD34), immune cells (CD45/PTPRC), T-cells (CD3, CD4, CD8, CD69 and CD103), monocytes/macrophages (CD11c and CD11d) and MHC molecules (MHC-II and MHC-I). c FeaturePlot representation of ADT protein expression values for selected markers from b highlighting the specificity of major lineage markers on RNA based clustering in a

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Genome Medicine

ISSN: 1756-994X

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