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Fig. 2 | Genome Medicine

Fig. 2

From: Single-cell sequencing of the small and AT-skewed genome of malaria parasites

Fig. 2

Sequencing statistics show benefits of optimized MALBAC. a Contribution of reads based on organism type.  A subset of 10,000 reads from each sample were randomly selected for BLAST to identify sources of DNA. Color representation: bacteria (red); human (blue); other organisms (orange); Plasmodium (grey). b GC content of P. falciparum mapped reads. GC content of reads was calculated by Qualimap. Color representation: EOM (grey): early-stage single parasites amplified by optimized MALBAC; LOM (purple): late-stage single parasites amplified by optimized MALBAC; ENM (orange): early-stage single parasites amplified by non-optimized MALBAC; LNM (dark red): late-stage single parasites amplified by non-optimized MALBAC; Dd2 bulk genomic DNA (black); COM samples (blue): clinical single parasites amplified by optimized MALBAC. Clinical bulk genomic DNA is not shown here due to < 1% of the genome being covered by at least one read. c Fraction of P. falciparum genome covered by at least 1 read. The fraction of the genome was calculated by Qualimap. Color representations are the same as described in panel b

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