Fig. 3

a Experimental schematic for slice culture drug screening (6 drugs, 2 controls) from a single patient (PW030). b Heatmap showing the number of differentially expressed genes (FDR<0.01) in the tumor, myeloid, and oligodendrocyte populations between treated and control slices for each drug in the screen illustrated in a. c Same as b but showing only differentially expressed genes with FDR<0.01 and fold-change amplitude greater than two (both up- and downregulated genes). d UMAP embedding of scRNA-seq profiles of transformed cells from the control slices colored by expression of two proliferation markers (TOP2A, MKI67), two mesenchymal markers (CD44, VIM), and an astrocyte marker (GFAP). e Same as d but with UMAP projection density of scRNA-seq profiles of transformed cell from the treated slice cultures for each drug. Note that there is negligible projection density for the etoposide-treated cells onto the control cells for the small proliferative population expressing TOP2A and MKI67