Fig. 9

Behavioral analyses of dhx30 mutant zebrafish. a Distance moved of dhx30 mutants and wild-type sibling controls measured at 5 days post fertilization. b Average of distance moved during 14-h daytime. c Average of distance moved during 10-h nighttime. N = 15, 18, and 25 for +/+, +/−, and −/− animals, respectively. d Social preference index (SPI) calculated during 10-min baseline and post-baseline period. SPI = 1 indicates a fish that spends 100% of its time near a conspecific, SPI = − 1 indicates a fish that spends 100% of its time near the empty well, and SPI = 0 indicates a fish that spends equal amounts of time near the conspecific and near the empty well. e The change in SPI between baseline and post-baseline, indicating the preference of zebrafish to stay close to conspecific fish. N = 13, 6, and 17 for +/+, +/−, and −/− animals, respectively. Data are presented as means ± standard error of mean based on the indicated number of embryos. *: significantly different from dhx30+/+ (*p< 0.05; unpaired Student’s t test)