Fig. 4

Dynamics of MARCO expression under PD1 immunotherapy. a Comparison of bulk MARCO expression in a longitudinal cohort of GBM patients treated with adjuvant PD1 checkpoint inhibitors following recurrence. Immunotherapy responders showed a decrease in MARCO expression between pre- and post-immunotherapy recurrences. b Timeline of representative examples of a responder (patient 55, blue) and non-responder (patient 20, orange) to PD1 immunotherapy. MARCO expression levels from available samples are plotted concurrently with the disease course. Each tick on the x-axis represents 1 month of time. c Immunofluorescence imaging of these same two representative cases (patients 55 and 20) before and after anti-PD-1 therapy, with dual staining of CD163 (green) and MARCO (red), alongside DAPI (blue)