Fig. 5

Selection of SARS-CoV-2 B cell epitope regions. A SARS-CoV-2 linear B cell epitopes curated from epitope mapping studies. X-axis represents amino acid position along the SARS-CoV-2 spike protein, with labeled start sites. B Schematic for filtering criteria of B cell epitope candidates. C Amino acid sequence of spike protein domains considered for B cell epitope selection, with overlay of selection features prior to filtering. Polymorphic residues are red, glycosites are blue, accessible regions highlighted in yellow. The receptor binding domain (RBD), fusion peptide (FP), and HR1 regions are outlined. HR2 excluded for lack of accessibility data. D Spike protein functional regions (RBD, FP, HR1) amino acid sequences, with residues colored by how many times they occur in identified epitopes. Selected accessible sub-sequences of known antibody epitopes highlighted in purple outline. E S protein trimer crystal structure with glycosylation, with final linear epitope regions highlighted by color