Fig. 8

Experimental assessment of T and B cell epitope immunogenicity. A Mice were vaccinated with sixteen predicted T cell and B cell epitopes, designated as “peptides,” in combination with poly(I:C), or with poly(I:C) alone. T cell activity in response to vaccination was measured via IFN-y ELISpot with splenocytes isolated from mice at experimental day 21, plated with individual peptides. Activity was calculated by ELISpot plate reader. Peptide designations indicate protein, start, and end as shown in Fig. 6B. B Antibody response against predicted B cell peptide epitopes was measured via peptide ELISA. Wells were coated with pairs of predicted B cell peptides. C Antibody response against S protein was assessed via whole protein ELISA. Response to bovine serum albumin (BSA) was measured as negative control. For all subfigures, asterisks indicate statistically significant p value (< 0.05) from Mann-Whitney U tests of poly(I:C) + peptide groups compared to poly(I:C) alone