Fig. 2

TRBV gene usage differs between CD4⁺ and CD8⁺T cell lineage and not by tissue or subset. A Heatmap of TRB gene usage of T cell clones from individual samples (columns) for D383, D324 and D466 (left to right). Each unique clone is counted once per donor. B Principle component analysis (PCA) of TRBV gene usage by clone counts per sample for D383. PCA plots are labeled and grouped by lineage, tissue, and subset with confidence ellipses plotted around group mean points using the factoextra R package. C Barplot showing the mean TRB gene usage of CD4⁺ and CD8⁺T cells across 11 individuals with error bars indicating ± standard error. (CD4⁺, N = 61; CD8⁺, N = 58). t test, ns: p > 0.05, *: p≤0.05, **: p≤0.01, ***: p≤0.001, ****: p≤0.0001