Fig. 3

A Observed richness between subjects with (n = 24) and without T2D (n = 24), boxplots with Tukey-whiskers and mean (◆) as well as median are shown. Unpaired samples Wilcoxon signed-rank test is used to compare groups and nominal p-value is shown. B Differentially abundant genera in subjects with T2D (n = 24) compared to subjects without T2D (n = 24) at baseline. Differential abundance is calculated at ASV level using taxonomy after stringent control for contamination at a decontam score of 0.5 and reported at the genus level. C Spearman’s rank correlations of relative selected genera abundance with host parameters at baseline. Selection included genera seen to be differentially abundant between groups (i.e., T2D, good/poor responders, and T2D alleviation vs no T2D alleviation). Only genera are shown with at least one significant correlation with host markers. (+) refers to p-value < 0.05. Color represents correlation strength (Rho) according to color legend. Abbreviations: HDL high-density lipoprotein, LDL low-density lipoprotein, WHR waist to hip ratio, BMI body mass index, CRP C-reactive protein, sCD14 soluble cluster of differentiation 14, LBP lipopolysaccharide binding protein, EBL excess BMI loss