Fig. 3

Ixabepilone induces apoptosis and decreases proliferation in MYC-amplified Gp3 MB. a Representative images of Ki67 and CC3 immunostaining as a marker of cell proliferation and apoptosis respectively in Med-211FH (a, left) and Med-411FH (b, left) subcutaneous tumours following vehicle (top panel) and ixabepilone (bottom panel). Scale bar 20 μm. Quantitative analysis of Ki67 and CC3 staining in Med-211FH (a, right) and Med-411FH (b, right) tumours. Percentage of tumour cells staining positive were quantified for vehicle (n = 3) and drug-treated tumours (n = 3) using Image J software. Data are presented as the mean ± SEM. Statistical evaluation was performed using a Holm-Sidak corrected multiple t test with statistically significant differences indicated (*P < 0.05, **** P < 0.0001)