Fig. 4
From: Mobilization of the nonconjugative virulence plasmid from hypervirulent Klebsiella pneumoniae
Mobilization of pRJF293HA with the help of the IncF plasmid pOX38-Gen. a XbaI PFGE and S1-PFGE of E. coli transconjugants and their parental strains. Full details of the strains are provided in Additional file 1: Table S1. Red triangles denote the virulence plasmid pRJF293HA. Blue triangles denote the IncF plasmid derivative pOX38-Gen. Yellow triangles denote the hybrid plasmid (full details are shown in Fig. 5). Strains with the same symbol on the PFGE image represent progeny derived from the same parental strain. b Schematic diagram of the conjugation assays. The red square denotes the hph tag on the virulence plasmid pRJF293HA. The green rectangle denotes the tra gene cluster coding for a T4SS on the IncF plasmid derivative pOX38-Gen. The orange triangles denote the specific 28-bp fusion site