Table 1 Conjugation frequency of the virulence plasmid pRJF293HA and its derivatives
From: Mobilization of the nonconjugative virulence plasmid from hypervirulent Klebsiella pneumoniae
Transconjugant | Donor | Recipient | Conjugation frequency |
|---|---|---|---|
RJBSI76-pVa (iroB+) | RJF293HA (pV-harbouring, iroB+) | RJBSI76 (p1-harbouring) | (1.01 ± 0.30) × 10−7 |
RJBSI76-pVC (iroB+, rmpA+) | C600-p1’-pVC (iroB+, rmpA+) | RJBSI76 | (5.59 ± 2.79) × 10−6 |
J53-pVb (iroB+) | RJBSI76-pV (iroB+) | J53 | (1.43 ± 0.74) × 10−4 |
J53-pVCc (iroB+, rmpA+) | RJBSI76-pVC (iroB+, rmpA+) | J53 | (3.03 ± 1.46) × 10−7 |
J53-p1-pV-hybrid-1 (iroB+) | RJBSI76-pV-hybrid (iroB+) | J53 | (4.58 ± 1.48) × 10−5 |
C600-p1-pV-hybrid (iroB+) | J53-p1-pV-hybrid-1 (iroB+) | C600 | (2.02 ± 0.22) × 10−1 |
C600-pVd (iroB+) | J53-p1’-pV-TC1 (iroB+) | C600 | (4.18 ± 1.47) × 10−2 |
J53-p1 | RJBSI76 | J53 | (2.38 ± 0.50) × 10−3 |
J53-p1’-pV-TC2e (iroB+) | RJF293HA (pV-harbouring, iroB+) | J53-p1 | (2.22 ± 0.45) × 10−7 |
C600-p1 | J53-p1 | C600 | (1.88 ± 0.56) × 10−2 |
C600-p1’-pV-TC2 (iroB+) | RJF293HA (pV-harbouring, iroB+) | C600-p1 | (5.53 ± 0.63) × 10−6 |
C600-p1’-pVC (iroB+, rmpA+) | RJF293C (pVC-harbouring, iroB+, rmpA+) | C600-p1 | (5.83 ± 3.49) × 10−8 |
J53-pF-pV-TC1 (iroB+) | XL10-pF | J53-pV (iroB+) | (4.19 ± 0.21) × 10−2 |
XL10-pF-pV-TC1f (iroB+) | J53-pV (iroB+) | XL10-pF | (1.04 ± 0.57) × 10−7 |
XL10-pVg (iroB+) | J53-pF-pV-TC1 (iroB+) | XL10 | (2.65 ± 1.33) × 10−6 |
XL10-pF-TC | J53-pF-pV-TC1 (iroB+) | XL10 | (2.71 ± 1.04) × 10−3 |
J53-pF-pV-TC2h(iroB+) | XL10-pF-pV-hybrid-1 (iroB+) | J53 | (6.74 ± 0.72) × 10−2 |
J53-pVWTi (iroB+, rmpA+) | RJF293-pF (pVWT-harbouring, iroB+, rmpA+) | J53 | (2.04 ± 0.36) × 10−6 |
HS11286-pF-pVWT-1j (iroB+, rmpA+) | J53-pF-pVWT-hybrid | HS11286-pKPHS2ΔoriT | (1.46 ± 0.21) × 10−5 |
HS11286-pF-pVWT-2k (iroB+, rmpA+) | J53-pF-pVWT | HS11286-pKPHS2ΔoriT | (1.68 ± 0.28) × 10−9 |
J53-pFΔoriT-p1l | XL10-pFΔoriT-p1 | J53 | (4.04 ± 0.52) × 10−7 |