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Fig. 3 | Genome Medicine

Fig. 3

From: Functional significance of gain-of-function H19 lncRNA in skeletal muscle differentiation and anti-obesity effects

Fig. 3

H19-GOF mutant promotes myotube differentiation and fusion. a Immunofluorescence staining using the indicated antibodies of iPSC-differentiated, H19-proficient or H19-deficient myotubes, with expression of backbone plasmid (Blank), H19 WT, or H19-GOF plasmid. Scale bars, 100 μm. b, c Statistical analysis of DMD (b) or MHC (c) staining intensities of iPSC-differentiated, H19-proficient or H19-deficient myotubes, with expression of indicated plasmid. Mean±SD, n = 6 independent experiments, one-way ANOVA. d Immunofluorescence staining using the indicated antibodies of iPSC-differentiated, H19-proficient or -deficient myotube fusion, with expression of indicated plasmid. Scale bars, 100 μm. (e) Percentage of fusion index of iPSC-differentiated, H19-proficient or H19-deficient myotube fusion, with expression of indicated plasmid. Mean ± SD, n = 6 independent experiments, one-way ANOVA. No significance [n.s.], p > 0.05, *, p < 0.05, **, p < 0.01, ***, p < 0.001

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Genome Medicine

ISSN: 1756-994X

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