Fig. 4
H19-Rgof mimics facilitate myotube differentiation and fusion. a Graphic illustration of the indicated RNA mimics predicted by RNAfold WebServer. b Competition binding assay to determine the Kd of the interaction between DMD (aa.3046–3685) and H19 WT, Rgof, or Rlof mimics. Unlabeled indicated RNA mimics served as competitors. Mean ± SD, n = 3 independent experiments. c Immunofluorescence staining using the indicated antibodies of iPSC-differentiated myotubes treated with Scr, H19-WT, H19-Rgof, or H19-Rlof mimics. Scale bars, 100 μm. d, e Statistical analysis of DMD (d) or MHC (e) staining intensities of iPSC-differentiated myotubes with indicated treatment. Mean±SD, n = 5 independent experiments, one-way ANOVA. f Immunofluorescence staining using the indicated antibodies of iPSC-differentiated myotubes with indicated treatment. Scale bars, 100 μm. g Percentage of fusion index of iPSC-differentiated myotubes with indicated treatment. Mean ± SD, n = 5 independent experiments, one-way ANOVA. No significance [n.s.], p > 0.05, *, p < 0.05, **, p < 0.01, ***, p < 0.001