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Fig. 6 | Genome Medicine

Fig. 6

From: Functional significance of gain-of-function H19 lncRNA in skeletal muscle differentiation and anti-obesity effects

Fig. 6

AGR-H19-Rgof alters muscle fiber type composition. a Immunofluorescence staining using the indicated antibodies or H&E staining of TA treated with AGR-Scr or AGR-H19-Rgof. Scale bars, 1 mm or 100 μm as indicated. b Number of muscle fibers per muscle piece of animals with indicated treatment. Mean ± SD, n = 5 animals per experimental group, Student’s t test. c Frequency distribution of cross-sectional TA-muscle fiber area. Mean ± SD, n = 5 animals per experimental group. d SDH staining (top) and immunostaining of different skeletal muscle fiber-types (bottom) from TA of animals with indicated treatment. Scale bars, 1 mm. e, f Percentage of different skeletal muscle fiber-types from QUAD (e) or SOL (f) of animals with indicated treatment. Mean ± SD, n = 5 animals per experimental group, Student’s t test. g SDH-positive fibers per muscle piece of animals with indicated treatment. Mean ± SD, n = 5 animals per experimental group, Student’s t test. h Forelimb grip strength test of male (left) or female (right) animals with indicated treatment. Mean ± SD, n = 8 animals per experimental group, Student’s t test. i Running speed of male (left) or female (right) animals with indicated treatment. Mean ± SD, n = 15 animals per experimental group, Student’s t test. j Top: graphic illustration of sprint treadmill protocol. Bottom: Maximum speed of male (left) or female (right) animals with indicated treatment. Mean ± SD, n = 10 animals per experimental group, Student’s t test

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