Fig. 4

Knockdown of TBX15 in human primary preadipocytes significantly affects 130 genes (FDR < 0.05) in the WHRadjBMI co-expression network. A Illustration of TBX15 gene with introns and exons; and the relative RNA-seq read density in the human primary preadipocyte cells transfected with the negative control siRNA when compared to the cells transfected with the TBX15 siRNA. Scales for the read density are equal. B Bar plot showing the qPCR relative expression (2^−ddCt) when compared to the housekeeping gene 36B4 and RNA-seq TPMs for TBX15 in the cells transfected with negative control siRNA when compared to the cells transfected with TBX15 siRNA (n = 5). C Volcano plot of differentially expressed (DE) genes in TBX15 knockdown experiment, excluding TBX15. Significant genes (FDR < 0.05) (dark gray), non-significant genes (light gray), and TFs (orange; FDR < 0.05) are plotted based on their log₁₀ p value and log₂ fold change in expression. Significantly differentially expressed TFs are labeled. Inlay shows the volcano plot of the TBX15 DE results with TBX15 included