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Fig. 2 | Genome Medicine

Fig. 2

From: Using single-nucleus RNA-sequencing to interrogate transcriptomic profiles of archived human pancreatic islets

Fig. 2

Side-by-side comparison of single-cell vs. single-nucleus RNA-sequencing in freshly cultured human islets. A Experimental design of the workflow for generating single-cell RNA-seq (scRNA-seq) and single-nucleus RNA-seq (snRNA-seq) datasets of cultured human islets. BD Violin plots representing B number of reads (UMI), C number of genes, and D proportion (expressed in %) of mitochondrial genes per cell/nucleus in scRNA-seq (orange violins) and snRNA-seq (blue violins) datasets. Data are expressed in logarithmic scale (Y-axis). Statistical significance was tested using a Wilcoxon rank-sum test. E, F UMAP plots representing the clustering of the high-quality E cells and F nuclei in the E scRNA-seq and the F snRNA-seq datasets. Y-axis of plot in F is like the plot in E. G Global integrated UMAP plot representing the distribution of high-quality cells (from scRNA-seq, orange dots) and nuclei (from snRNA-seq, blue dots)

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