Fig. 1

Differential DNA methylation and related pathways in MCC. A Illumina Infinium MethylationEPIC BeadChip revealed DNA methylation profiles in the four groups (Cluster 1-4) by comparing Normal, Primary, Metastatic_Sk (Sk for skin), Metastatic_LN (LN for Lymph Node). DNA methylation in these groups was compared using ANOVA. Using P < 0.05 and SD of the mean > 0.25, we obtained 24,497 probes. Using hierarchical clustering of probes, four clusters were identified B PCA plot showing that the selected probes separated samples in three groups: Normal, Metastatic lymph node (LN), and Primary and Metastatic_Sk. C Differentially methylated genes in clusters 1-4 were annotated using GO terms for biological process. GO analysis for biological terms using the R function RDAVID was performed and GO terms were reported