Fig. 2

DNA methylation differences based on MCPyV status in MCC. A Comparison of DNA methylation in MCPyV-positive vs MCPyV-negative samples using Student’s t test revealed 470 loci that were differentially methylated FDR-adjusted p < 0.05 and minimum delta change of ± 0.4. Heatmap shows DNA methylation profiles in MCC tumor, adjacent-normal skin, and normal lymph node samples. B These probes characterized samples in MCPyV-positive (red/orange) and MCPyV-negative (pink/sky blue) groups in primary (red/ pink), metastatic skin (orange), and metastatic lymph node (sky blue) samples. Adjacent-normal skin (forest green and light green), normal lymph node (green) samples based on PCA plot. C Mean DNA methylation from selected loci differentially methylated in MCPyV positive and MCPyV negative samples based on hyper or hypomethylated in MCPyV positive and MCPyV negative is recorded and depicted in bar-graphs. Adjusted p value (FDR) significance thresholds are indicated: 0.01 < p < 0.05 (*); 0.001 < p < 0.01 (**); p < 0.001 (***)