Fig. 4
Expression of 5A and 5N in the mouse cortex across development. A The expression of 5A (red) in Scn1a is shown for 58 mouse cortex samples across development (points); no functional 5N equivalent is present in the mouse genome. On the left, the colored line shows the Loess smoothed average and 95% confidence interval (shaded region). On the right, boxplots show the median and interquartile range for the same data, binned into fetal, transitional, and mature developmental stages. B The Loess smoothed average expression of the four voltage-gated sodium channels in human cortex (top, Fig. 1) and mouse cortex (bottom). C Panel A is repeated for Scn2a, with the addition of 5N expression (blue). D The ratio of 5A and 5N expression from panel ‘C’ is shown across development (left) and in three developmental stages (right). Values reported previously in mouse cortex are shown on the same scale in green for comparison [20]. E-H Panels C and D are repeated for the genes Scn3a, Scn8a. CPM: Counts per million. Statistical tests: D, F, H Left panel, linear regression of log2(5A:5N ratio) and log2(post-conceptual days). Right panel, two-tailed Wilcoxon test of log2(5A:5N ratio) values between fetal and mature groups