Fig. 6

Identification of protein-coding exons with complex developmental trajectories. A The correlation between the ratio of CPM expression between pairs of exons (log-scaled) and developmental stage (post-conceptual days, log-scaled) for SCN1A was assessed with a linear model (e.g., Fig. 2B). The R² value of each exon pair is shown as a heat map with ‘hot’ colors representing exon pairs with high R² values for which variation in the ratio is correlated with developmental age, i.e., pairs of exons that show substantially different expression across development. Exon numbers from DEXSeq (Additional file 3: Table S2) are shown on the bottom and right and equivalent CDS numbers are shown on the top and left (see Additional file 3: Table S2). B–D The analysis is repeated for SCN2A, SCN3A, and SCN8A