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Fig. 1 | Genome Medicine

Fig. 1

From: Multi-omics of the esophageal microenvironment identifies signatures associated with progression of Barrett’s esophagus

Fig. 1

Host transcriptome changes in the esophagus of patients with GERD and metaplasia. Shotgun sequencing of ribosomal-depleted RNA was performed on a NovaSeq platform. A Changes in predicted abundance of resting mast cells across the different groups. B Changes in predicted abundance of active mast cells across the different groups. Abundances were calculated using CIBERSORTx and statistical differences were tested using Kruskal-Wallis with Dunn’s multiple comparisons test. C Volcano plot of differentially expressed genes between NORM and GERD samples. D Volcano plot of differentially expressed genes between NORM and MET samples. Differential expression was calculated using DESeq2. Red, upregulated in disease state, Green, downregulated in disease state. E Genes consistently regulated across GERD and MET when compared to NORM samples. Scale bar is fold-change. F Pathway analysis of differentially expressed genes in MET samples. G Tissue type and disease analyses of differentially expressed genes in MET samples. All analyses were conducted using Enrichr. H Significant changes in the host esophageal transcriptome occur at the time of metaplasia

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