Fig. 4

Characteristics of C. concisus ESOS44-1 and ESOS44-4 compared to other C. concisus isolates from this cohort. A Circular representation of the 10 C. concisus genomes using GView. ESOS44-1, red; other isolates, green. Region I: Prophage CP4-57; Region II: lipid A biosynthesis. B Syntenic proteins unique to ESOS44-1 as compared to other isolates, including LprI_01601. C Susceptibility of representative Campylobacter isolates to lysozyme as well as lysozyme and lactoferrin. Experiments were repeated in triplicate and representative results were presented. D Intracellular levels of representative wild-type Campylobacter isolates and the ΔLprI_01601 mutant within primary human macrophages. Levels were calculated using a gentamicin protection assay at MOI 100. ESOS44-1 was significantly different to all other strains except for ESOS44-4 (ANOVA, Dunnett’s; * P < 0.05, ** P < 0.01). Decreased intracellular levels of ESOS44-4 ΔLprI_00928 as compared to the wild-type isolate were validated in primary macrophages from another donor (Welch’s t-test; * P < 0.05). E Acidification of lysosomes following infection with wild-type C. concisus isolates and the mutant strain. Fluorescence was measured following the addition of LysoTracker Green. ESOS44-1 and the ΔLprI_01601 mutant were both significantly different to the negative control (ANOVA, Dunnett’s; ** P < 0.01, *** P < 0.001). F Experimental procedure for LprI_01601 recombinant expression and immunoprecipitation following incubation with primary macrophage lysates. G Human proteins that co-immunoprecipitated with LprI_01601. Proteins are arranged according to combined mascot score from two IP and LC/MS-MS experiments. H Prevalence of LprI_01601 in shotgun metagenomics data of esophageal mucosal brushings from patients in the early stages of the EAC cascade. Reads aligning to LprI_01601 within the data were identified using BWA-MEM