Fig. 3
From: The expressed mutational landscape of microsatellite stable colorectal cancers
Mutant allele-specific expression levels vary by mutation type and target gene. a Scatter plot of MAFs in the RNA versus exome sequencing data of one selected tumor. The dashed line indicates expected expression levels according to the allelic frequency. A few target genes with preferential expression of the mutated alleles, including SMAD (three separate mutations) and KRAS, contribute to the weak statistical correlation between the RNA-level and DNA-level MAFs. The difference between the RNA-level and DNA-level MAFs (ΔMAF RNAadjusted|DNA) of expressed mutations in b the in-house tumor series and c the validation series from TCGA is plotted separately for mutations in oncogenes/tumor suppressor genes (as designated in the Cancer Gene Census) and other genes and grouped according to mutation types (color-coded). Only mutation types with ≥ 10 mutations are plotted, and the total numbers of mutations per mutation type are indicated. ΔMAF RNAadjusted|DNA above or below 0 indicate mutated loci with preferential expression of the mutated or wild-type alleles, respectively