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Fig. 1 | Genome Medicine

Fig. 1

From: De novo transcriptomic subtyping of colorectal cancer liver metastases in the context of tumor heterogeneity

Fig. 1

Comparison of gene expression profiles of CRLMs with normal liver tissue samples, primary CRCs, CRC cell lines, and PDOs. a PCA showed sample clustering based on sample type and tissue of origin. CRLMs had largest variation along PC1, as indicated by the density plot on top. b PC1 versus sample-wise liver scores calculated by GSVA of a set of genes highly expressed in the liver. The liver scores of CRLMs ranged from the normal liver tissue samples to the primary CRCs. 27% of the CRLMs had a liver score below the maximum score for primary CRCs, as indicated by the gray dashed line. c Repeated PCA plot of all samples along the PC1 and PC2 axes, colored according to the microarray expression levels of ALB and KRT20. The tree CRLM samples that clustered close to non-malignant liver samples in part a were excluded. d Hierarchical clustering of multiple (two to eight) distinct CRLMs from each of 45 patients. The tree is divided into five main branches, denoted A–E. Patients (n = 13) with adjacent clustering of all metastases are marked with black dots. Patients (n = 28) with separation of metastases into two or more main branches are represented by unique patient-wise colors. Patients (n = 4; P16, P21, P23, P45) with all metastases clustering within the same main branch, although not adjacent to each other, are colored gray. Three selected patients (P8, P10, P39) with separation of metastatic lesions on 2–3 of the main branches each are emphasized with arched lines

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