Fig. 5

LMS predictions and intra-patient heterogeneity in additional CRLM samples. a Subtype distributions from LMS predictions in two publicly available datasets of resected CRLMs, also according to available clinical information. b GSEA results for selected signatures in each external series corresponded fairly well with the patterns observed in the in-house series. c Left panel: LMS predictions of multiple CRLM samples from a subset of patients in the in-house series. Each horizontal bar represents a patient, categorized according to heterogeneous or homogenous LMS classifications, and the length of the bars corresponds to the number of samples analyzed. Multiregional samples analyzed from each of 15 metastatic lesions are separated by white diagonal lines. Samples/lesions from repeated hepatic resections of seven patients are indicated with a black outline, including one patient with three resections. Right panel: the pie chart summarizes the proportion of overall intra-patient inter-metastatic subtype heterogeneity among the 42 patients with multiple metastatic lesions from the same hepatic resection. The bar plot below shows the proportion per LMS group and is calculated patient-wise among all patients who have at least one metastatic lesion/sample (from the same resection) classified in the specified subtype