Fig. 2

Comparison with conventional methods. a Comparison with PCR genotyping for mono- or tetranucleotide repeat microsatellites. For each of 46 CRCs, the fraction of unstable loci was plotted. For PCR genotyping assays, five microsatellite markers were used for each. For the sequencing assay, 144 mono- and 38 tetranucleotide repeat microsatellites were used, respectively. b and c Comparison with digital PCR (dPCR) and whole genome sequencing (WGS) for gene copy numbers. For each of 46 CRCs, the log2 gene copy number ratio between tumor versus matched normal samples was plotted. To validate the sequencing results, seven genes (VEGFA, MET, FGFR1, CDK4, FLT3, ERBB2, and AURKA) were selected for dPCR testing. For comparison with WGS, 83 target genes were used. In all the plots, dotted black lines indicate linear regression, and the correlation is indicated as an R-squared value