Fig. 5

Clonal diversity of hypermutated tumors. a Clonal diversity analysis for hypermutated tumors. Relative cellular prevalence of each clone, indicated by overlapping bars in the plot, was estimated by PyClone based on the allelic fraction of the mutations shared by each clonal population. The number of mutations for each clone is indicated on the inside of each bar. b Clonal diversity shown in MSI analysis. For a microsatellite locus (NR-21), allele profiles of both normal and tumor samples from P1595 are shown. Electropherograms generated by the PCR-CE method (left panels) and the allele histograms generated by our deep sequencing approach (right panels) are compared. For both panels, x- and y-axes indicate individual microsatellite alleles and their relative abundance, respectively. From the tumor allele profile, many different allelic shifts are observed