Fig. 4

Single-cell mutation profile of matched primary and metastatic tumours. A Dot plots show multidimensional scaling analysis of the single-cell mutation profile, where each dot represents a single cell and is grouped by hierarchical clustering. Cells are colour-coded based on the sampling location. B Heatmap shows the single-cell somatic SNV profiles of CRC5. All single cells are in rows and sorted according to hierarchical clustering. SNVs are hierarchically clustered in columns. Red bars represent mutations, grey bars represent reference alleles, and white bars represent sites with a low sequencing depth (NA). Each SNV cluster is colour-coded as described in the legends. Genes with high-risk mutation (SIFT score < 0.05, POLYPHEN >0.85) were labelled in red