Fig. 6

Mutation frequencies and functional validation of metastatic tumour cell-specific mutated genes. A, B Bar plots on the left panel show the comparison of mutated genes with significantly different mutation frequencies between metastatic tumour cell clones and primary tumour cell clones in CRC4 (A) and CRC7 (B). Fold change values are represented on the log2 scale. Primary and metastatic tumour cell clone-specific-mutated genes are colour-coded in blue and red, respectively. * represented mutation with SIFT <0.05, POLYPHEN >0.85. The heatmaps in the right panel show the gene mutation profiles of metastatic tumour cell clones and primary tumour cell clones from CRC4 (A) and CRC7 (B). C Real-time impedance traces for 4 CRC cell lines transfected with an siRNA against DNAH3 (colour-coded in red), the control siRNA (colour-coded in blue) and the blank group (colour-coded in green). The name of each cell line is labelled on the top of each panel