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Table 1 Clinicopathological patient characteristics of the gastric cancer test cohort consisting of nine men (mean age 68 years; range 50–85 years)

From: Multiscale heterogeneity in gastric adenocarcinoma evolution is an obstacle to precision medicine

Case Case #1 Case #2 Case #3 Case #4 Case #5 Case #6 Case #7 Case #8 Case #9
Localization Cardia Cardia Antrum/Corpus Antrum Fundus Cardia Cardia Cardia Antrum
Neoadjuvant treatment Yes Yes No No No No Yes No Yes
Laurén phenotype Intestinal Mixed Mixed Mixed Diffuse Intestinal Intestinal Mixed Diffuse
Tumor size [cm] 3.8 4.4 12.9 4.0 4.8 4.1 6.7 6.2 3.7
Epstein-Barr virus status Negative Negative Negative Negative Negative Negative Negative Negative Negative
Microsatellite status MSS MSS MSS MSS MSS MSI MSS MSS MSS
HER2 status Negative Negative Positive Positive Negative Negative Negative Negative Negative
pT category ypT3 ypT3 pT3 pT3 pT3 pT3 ypT3 pT2 ypT3
pN category ypN1 (1/29) ypN1 (2/51) pN3a (13/17) pN2 (3/19) pN3b (28/46) pN0 (0/19) ypN0 (0/12) pN0 (0/25) ypN2 (4/22)
pM category X X X X X X X X X
Grading n.a. n.a. G3 G3 G3 G2 n.a. n.a. n.a.
Number of tumor samples sequenced 4 3 6 5 10 5 4 6 5
Non-synonymous mutations (total valid) 184 373 714 348 425 3111 181 369 242
Copy number variations (number of genes) 6 22 65 11 98 3 5 1 0
Tumor sample Number/percentage of non-synonymous mutations present only in a single sample
 1 sample 71 (38.6%) 159 (42.6%) 350 (49.0%) 133 (38.2%) 211 (49.6%) 1262 (40.6%) 112 (61.9%) 235 (63.7%) 140 (57.8%)
  Number/percentage of same non-synonymous mutations present in ≥ 2 samples
 2 samples 25 (13.6%) 59 (15.8%) 78 (10.9%) 15 (4.3%) 29 (6.8%) 360 (11.6%) 24 (13.3%) 25 (6.8%) 30 (12.4%)
 3 samples 58 (31.5%) 155 (41.6%) 31 (4.3%) 11 (3.2%) 27 (6.4%) 180 (5.8%) 18 (9.9%) 9 (2.4%) 23 (9.5%)
 4 samples 30 (16.3%)    8 (1.1%) 26 (7.5%) 17 (4.0%) 148 (4.8%) 27 (14.9%) 14 (3.8%) 28 (11.6%)
 5 samples      25 (3.5%) 163 (46.8%) 13 (3.1%) 1161 (37.3%)    17 (4.6%) 21 (8.7%)
 6 samples      222 (31.1%)    18 (4.2%)      69 (18.7%)   
 7 samples          24 (5.6%)         
 8 samples          15 (3.5%)         
 9 samples          25 (5.9%)         
 10 samples          46 (10.8%)         
Per patient Clonality
 Clonal (non-synonymous) 2 (2.0%) 80 (50.0%) 62 (23.9%) 1 (0.9%) 1 (1.3%) 0 (0%) 0 (0%) 0 (0%) 0 (0%)
 Subclonal (non-synonymous) 98 (98.0%) 80 (50.0%) 197 (76.1%) 110 (98.2%) 76 (98.7%) 1083 (100%) 48 (100%) 81 (100%) 106 (100%)
 Not assessable (non-synonymous) 0 (0%) 0 (0%) 0 (0%) 1 (0.9%) 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%)
Total (non-synonymous) 100   160   259   112   77   1083   48   81   106  
Clonal (synonymous) 1 (6.0%) 32 (15.9%) 26 (10.1%) 2 (1.3%) 1 (5.7%) 0 (0%) 1 (0.4%) 0 (0%) 0 (0%)
Subclonal (synonymous) 159 (94.0%) 168 (83.6%) 231 (89.9%) 157 (98.7%) 174 (99.4%) 825 (97.6%) 229 (99.6%) 168 (100%) 257 (99.6%)
Not assessable (synonymous) 0 (0%) 1 (4.9%) 0 (0%) 0 (0%) 0 (0%) 2 (2.4%) 0 (0%) 0 (0%) 1 (0.4%)
Total (synonymous) 160   201   257   159   175   827   230   168   258