Fig. 4

Clustering of DAEs unravels temporal dynamics of brain gene regulation. A Heatmap displaying all available epigenome features for PCW 8-12, across all DAEs interacting with protein-coding genes in CP (upper heatmap) and GZ (lower heatmap) (A^I). K-means clustering analysis of epigenome features (A^II) identifies two clusters, cluster 1 (red) and cluster 2 (green). Level of enrichment is indicated on the y-axis in Log2 TPM. Box plots (A^III) shows RNA-seq gene expression of protein-coding genes regulated by the DAEs from each cluster (Expression pattern), for available data from PCW 8, 9, and 12 [20]. Boxes are IQR; line is median; and whiskers extend to 1.5 the IQR. Gene enrichment analysis for the corresponding genes in each cluster (A^IV). X-axis shows the − log 10 (p value) from Enrichr. B As for A, but now for PCW 13–18. C As for A, but now for PCW > 18