Fig. 4
Identification of Aβ-aggregated proteins through comparison of mRNA and protein levels. a Spearman’s rank correlation of significantly altered proteins with respect to their mRNA expression in the indicated comparisons. Bar colors indicate the mean half-life of the proteins as defined by [110]. b Discordant upregulation of GO biological processes and Reactome pathways at protein level that is not explained by the upregulation of the same genes at mRNA level. c Scatter plot depicting the logarithm of the fold change (LogFC) of mRNA (X-axis) and protein (Y-axis) for the AppNL-G-F vs. Appwt comparison at 6 mo. Proteins whose LogFC(protein) > 0.25 and their corresponding LogFC(mRNA) > 0.5 are shown in orange while those with a LogFC(protein) > 0.25 and LogFC(mRNA) < 0.5 are shown in red. Dot size is proportional to the negative logarithm of the adjusted p value. d Syt11 protein (continuous line) and mRNA (dashed line) levels at different time points relative to the Appwt at 3 mo. are shown for the AppNL-G-F (strong blue), AppNL-F (medium blue), and Appwt (corresponding to the AppNL-F model; light blue) mice. N = 4. e Representative microphotographs of the hippocampus of brain sections from 3-, 6-, and 9-mo. Appwt (top row) or AppNL-G-F (bottom row) mice stained with an anti-Syt11 antibody (green) and Hoechst dye (blue) (n = 3 for each condition). Scale bars represent 100 μm. f Representative micrographs of a brain section of a 6-mo. AppNL-G-F mouse stained with an anti-Syt11 antibody (green), the anti-Aβ antibody 6E10 (red), and Hoechst dye (blue). Scale bars represent 100 μm (n = 3)