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Fig. 4 | Genome Medicine

Fig. 4

From: Integrating thousands of PTEN variant activity and abundance measurements reveals variant subgroups and new dominant negatives in cancers

Fig. 4

PTEN variants and phospho Akt1. WT or PTEN variants were overexpressed in HEK 293T cells, lysed, and Western blotted for the HA tag (A, top), Beta-actin (A, bottom), Thr308 phosphorylated Akt1 (B, top), or all Akt1 (B, bottom). Representative Western blots are shown. Normalized band intensities of each of three independent Western blotting experiments are shown as points, while the mean value is shown as a black bar. C Scatterplot comparing the mean normalized pThr308 value to the number of independent samples each variant was observed in the cancer genomics databases. D Comparison of PTEN protein abundance level and pThr308 Akt1 abundance level calculated by reverse-phase protein array in the cancer cell line encyclopedia. Leu42Arg, which was not tested in our assay, is highlighted in blue as it exhibits strong reverse-phase protein array data for both PTEN abundance and pThr308 in the CCLE data

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Genome Medicine

ISSN: 1756-994X

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