Fig. 1

Characterization of SARS-CoV-2 natural infection-induced RBD-specific mAbs. a Antibody binding activity (EC₅₀) with native and denatured SARS-CoV-2 RBD as well as native RBD of SARS-CoV was measured by ELISA. Upper panel, four ranges per donor; lower panel, percentage of mAbs from each donor with the indicated EC₅₀ range. N.B., non-binding activity. b SARS-CoV-2 pseudovirus neutralization potency (IC₅₀) (left). Percentage of antibodies with indicated neutralization potencies (right). Results are derived from a single experiment performed in triplicate. c ACE2 blocking activity (IC₅₀) (left). Percentage of antibodies with indicated receptor-blocking potencies (right). For b and c, N.N., non-neutralization or non-blocking activity. The data represent one representative experiment of two independent experiments. d Distribution of heavy-chain variable (VH) germline genes of RBD-specific NAbs