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Fig. 2 | Genome Medicine

Fig. 2

From: Comprehensive mapping of binding hot spots of SARS-CoV-2 RBD-specific neutralizing antibodies for tracking immune escape variants

Fig. 2

Determination and characterization of the antigenic sites on RBD. a Mapping of binding sites of a panel of RBD-specific NAbs by global alanine scanning. Thirty-three amino acid positions were identified in four antigenic sites (1–4) on RBD as main targets for RBD-specific NAbs. Degree of binding reduction was defined as percentage by OD450 of each mutant relative to OD450 of RBD wildtype and is represented as a heatmap from white (reduction) to blue (no impact). The data are representative of at least two independent experiments. b Location of four distinct antigenic sites on the RBD region (PDB ID: 6M0J). The color-coding scheme is described as follows: site 1 (orange), site 2 (green), site 3 (slate blue), site 4 (red); ACE2 (wheat). Top and down are shown from different angles. The key hot spots targeted by NAbs are shown. c Key residues for VH3-53/3-66 dominant NAb recognition. Binding fold change was calculated as follows: EC50 of RBD mutant/EC50 of RBD wildtype. d Antibodies are grouped according to neutralization potency and colored by the usage frequency of each antigenic site. Each antibody was tested for competition with a panel of known antibodies and assigned to an antigenic site based on the competition profile. e Percentage of SARS-CoV-2 and SARS-CoV crossing reactive antibodies targeting each antigenic site. The data represent one representative experiment of two independent experiments

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